cDNA preparation
Two micrograms of total RNA is primed with random primer oligonucleotides and reverse-transcribed by MMLV reverse transcriptase in 20 µl final volume. The RT reaction is stopped by heating at 65°C for 10 minutes.
The cDNA is delivered in 1x RT buffer. (1x RT Buffer: 50 mM Tris-HCl, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT).
1 µl cDNA is sufficient for one PCR reaction.
Quality Control
Human beta-actin has been amplified by PCR.
Product Use
For in vitro laboratory reaserch use only.