The retinal pigment epithelium or RPE, is a single layer of cells in the eye. The retina consists of photoreceptors and retinal pigment epithelial cells (RPEpiC). RPEpiC are situated between the neurosensory retina and the choroid.
RPEpiCs have specific polarized protein distributions within the cell. In contrast to other epithelia which normally faces an apical lumen devoid of matrix, the RPEpiC apical plasma membrane is in direct contact with the extracellular matrix.
RPEpiC play a critical role in regulating the adjacent photoreceptor membrane turnover, retinoid metabolism, and protecting the retina from light-induced damages by the intracellular melanin.
Human Retinal Pigment Epithelial Cells (HRPEpiC) are isolated from human retina. HRPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 500 000 cells in 1 ml volume.
HRPEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18, -19 and fibronectin.
HRPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HRPEpiC are guaranteed to further expand for 10 population doublings under the conditions provided.
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. SC4101) for culturing HRPEpiC in vitro.
HRPEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.