Oligodendrocyte precursor cells are found in both the developing and adult central nervous system. Oligodendrocytes are the myelin-forming cells of the central nervous system and are differentiated from Oligodendrocyte precursor cells .
Oligodendrocyte precursor cells can be generated from neural progenitors or neural stem cells in the presence of basic fibroblast growth factor in vitro. OPCs proliferate in the presence of platelet-derived growth factor or factors produced by astrocytes and differentiate into mature oligodendrocytes.
HOPC are isolated from human brain. HOPC are cryopreserved after purification and delivered frozen.
Each vial contains 1 million cells in 1 ml volume.
HOPC are characterized by immunofluorescence with antibodies specific to A2B5 and O4.
HOPC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HOPC are guaranteed to further culture under the conditions provided; however, HOPC are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Oligodendrocyte Precursor Cell Medium (OPCM, Cat. no. SC1601) for culturing HOPC in vitro and Oligodendrocyte Precursor Cell Differentiation Medium (OPCDM, Cat. no. SC1631) for differentiating HOPC.
Product Use
HOPC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.