The tissue of the central nervous system is made up neurons and glia cells. Neurons are anatomic, functional, and trophic units of the brain. Despite great variability in size and shape, all neurons share common morphologic features as highly complex network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system. The brain is known to contain about 1 x 10^11 neurons, each being able to contact at least 10,000 other neurons.
Brain stem neurons provide main motor and sensory innervation and integrative functions by regulating heart rate, breathing, sleep cycle, awareness, consciousness, and more.
Human Neurons-brain stem (HN-bs) are isolated from human brain stem and are cryopreserved at P0.
Each vial contains 1 million cells in 1 ml volume.
HN-bs are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and β-tubulin III.
HN-bs are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HN-bs are guaranteed to further culture under the conditions provided
However, HN-bs are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
It is recommended to use neuronal medium (NM, Cat. SC1521) for culturing HN-bs in vitro.
HN-bs are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.