Meningeal cells surround the brain and actively participate in the development of the central nervous system.
They play an important role in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, and laminating the cerebellar cortex. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerebellar cortex and dentate gyrus. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex, in adult rat spinal cord lesions promotes axonal regrowth. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells.
Human Meningeal Cells (HMC) are isolated from human leptomeninges. HMC are cryopreserved at P1 and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HMC are characterized by immunofluorescence; they are positive for fibronectin and negative for GFAP, α-smooth muscle actin, and Thy 1.1.
HMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HMC are guaranteed to further expand for 15 population doublings under the conditions specified.
It is recommended to use Meningeal Cell Medium (MCM, Cat. SC1401) for culturing HMC in vitro.
HMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.