Central nervous system pericytes are perivascular cells that are closely associated with the endothelium of capillaries and other small vessels.
Pericytes, located near endothelial cells, communicate with other cells by extending long cytoplasmic processes which wrap around the capillaries.
Pericytes participate in a variety of processes including angiogenesis, endothelial cell survival, regulation of capillary blood flow, and establishment and maintenance of the bloodbrain barrier.
Leptomeningeal pericytes (LP) are derived from the leptomeningeal layer surrounding the brain. Human Leptomeningeal Pericytes (HLP) from ScienCell Research Laboratories are isolated from human brain. HLP are cryopreserved at passage 1 after purification and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HLP are characterized by immunofluorescence with antibody specific to a-smooth muscle actin and platelet derived growth factor-beta (PDGF-beta).
HLP are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HLP are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
It is recommended to use Pericyte Medium (PM, Cat. SC1201) for culturing HLP in vitro.
HLP are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
 Dore-Duffy P, Cleary K. (2011) "Morphology and properties of pericytes." Methods Mol Biol. 686:49-68.
 Allt G, Lawrenson JG. (2001) "Pericytes: cell biology and pathology." Cells Tissues Organs. 169: 1-11.
 Daneman R, Zhou L, Kebede A, Barres B. (2010) "Pericytes are required for blood-brain barrier integrity during embryogenesis." Nature. 468:562-566.
 Kutcher M, Herman I. (2009) "The pericyte: cellular regulator of microvascular blood flow." Microvasc Res. 77:235-246.