Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. Fibroblasts in the vascular adventitia, the outermost connective tissue covering of a vessel, have been implicated in vascular injuries through transient changes in their phenotypic and proliferative properties, and accumulation of extracellular matrix proteins in the adventitia . Such changes play an important role in the remodeling of vessel walls under pathological conditions, and thus suggest great potential for site-specific vascular wall gene therapy to regulate vascular tone through vascular adventitial fibroblasts [2, 3].
Human Brain Vascular Adventitial Fibroblasts (HBVAF) from ScienCell Research Laboratories are isolated from human brain.
HBVAF are cryopreserved at passage 1 and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HBVAF are characterized by spindle morphology and by immunofluorescence with antibody specific to fibronectin.
HBVAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast,and fungi.
HBVAF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
It is recommended to use Fibroblast Medium (FM, Cat. no. SC2301) for culturing HBVAF in vitro.
HBVAF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
 Shi Y, Pieniek M, Fard A, O'Brien J, Mannion JD, Zalewski A.(1996) "Adventitial remodeling after coronary arterial injury." Circulation. 93:340-8.
 Tsutsui M, Chen AF, O'Brien T, Crotty TB, Katusic ZS.(1998) "Adventitial expression of recombinant eNOS gene restores NO production in arteries without endothelium." Arterioscler Thromb Vasc Biol.18:1231-41.
 Kullo IJ, Mozes G, Schwartz RS, Gloviczki P, Crotty TB, Barber DA, Katusic ZS, O'Brien T.(1997) "Adventitial gene transfer of recombinant endothelial nitric oxide synthase to rabbit carotid arteries alters vascular reactivity." Circulation.96:2254-61.