The tonsils are the immune system’s first defense against foreign pathogens and help to prevent respiratory and gastrointestinal infections.
Tonsil endothelial cells (TEC) line the blood vessels and actively participate in fluid balance, cellular transport, and immunity [1]. TEC also perform specialized functions for the tonsils including recruiting lymphocytes from the blood [2, 3]. Studies have shown that TEC express proteins that aid in lymphocyte adhesion and extravasation [3].
HTEC are a useful model for studying inflammation in the tonsils.
Human Tonsil Endothelial Cells (HTEC) are isolated from human tonsil. HTEC are cryopreserved at passage one and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HTEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM).
HTEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HTEC are guaranteed to further expand for 10 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. No. SC1001) for culturing HTEC in vitro.
Product Use
HTEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
References
[1] Kelley P, Tempero R. (2010) "Lymphatic vessel hypertrophy in in inflamed human tonsils." Lymphat Res Biol. 8(2): 121-126.
[2] Garrafa E, Alessandri G, Benetti A, Turetta D, Corradi A, Cantoni A, Cervi E, Bonardelli S, Parati E, Giulini S, Ensoli B, Caruso A. (2006) "Isolation and characterization of lymphatic microvascular endothelial cells from human tonsils".J Cell Physiol. 207(1): 107-113.
[3] Palmeri D, Zuo F, Rosen S, Hemmerich S. (2004) "Differential gene expression profile of human tonsil high endothelial cells: implications for lymphocyte trafficking." J Leukoc Biol. 75(5): 910-927.