HMMEC are isolated from human uterus. HMMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM), and by uptake of DiI-Ac-LDL. HMMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMMEC are guaranteed to further expand for 10 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Endothelial Cell Medium (ECM, Cat. no. SC1001) for culturing HMMEC in vitro.
Product Use: HMMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Gargett C, Bucak K, Rogers P. (2000) “Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells.” Hum Reprod. 15(2): 293-301.
[2] Hayman R, Brockelsby J, Kenny L, Baker P. (1999) “Preeclampsia: the endothelium, circulating factor(s) and vascular endothelial growth factor.” J So Gynecol Investig. 6(1): 3-10.
[3] Weston G, Cann L, Rogers P. (2003) “Myometrial microvascular endothelial cells express oxytocin receptor.” BJOG. 110(2):149-156.