The lymphatic system is an essential part of the immune system. It helps maintain tissue homeostasis, including interstitial protein transport, tissue fluid balance, and development of cellular immunity. Lymph nodes, which are located throughout the lymphatic system, contain large numbers of lymphocytes, macrophages and antigen presenting cells that together initiate primary immune response. Specialized lymphatic endothelial cells (LEC) in the cortex assist in the primary immune response by recruiting intravascular lymphocytes as they circulate past.
Human Primary Lymphatic Endothelial Cells (HLEC) are isolated from human lymph node.
Human Lymphatic Endothelial Cells are cryopreserved at passage 1 and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
Human Lymphatic Endothelial Cells (HLEC) are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM), and by uptake of DiI-Ac-LDL.
Human Lymphatic Endothelial Cells (HLEC) are QA tested and found negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
Human Lymphatic Endothelial Cells (HLEC) are guaranteed to further expand for 10 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. no. SC1001) for culturing HLEC in vitro.
Product Use
HLEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.