The dura mater is the outermost layer of the meninges and functions to carry blood from the brain to the heart and contain the cerebrospinal fluid. The dura mater is composed of two layers known as the periosteal and meningeal layer.
Endothelial cells regulate blood vessel function and serve as a selective barrier for the diffusion of cells and macromolecules into and out of the bloodstream.
In addition, endothelial cells play an important role in vascular signaling, tone, remodeling, and inflammation. Dural microvascular endothelial cells (DuMEC) have been linked to migraine pathophysiology by activating and sensitizing meningeal afferents.
Human Dural Microvascular Endothelial Cells (HDuMEC) from ScienCell Research Laboratories are isolated from human dura mater. HDuMEC are cryopreserved at passage one and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HDuMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM).
HDuMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HDuMEC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
It is recommended to use Endothelial Cell Medium (ECM, Cat. no. SC1001) for culturing HDuMEC in vitro.
HDuMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen, and keep the cells in liquid nitrogen until they are needed for experiments.
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 Jacobs B, Dussor G. (2016) "Neurovascular contributions to migraine: Moving beyond vasodilation." Neuroscience 338:130-144.
 Tischfield M, Robson C, Gilette N, Chim S, Sofela F, DeLisle M, Gelber A, Barry B, MacKinnon S, Dagi L, Nathans J, Engle E. (2017) "Cerebral Vein Malformations Result from Loss of Twist1 Expression and BMP Signaling from Skull Progenitor Cells and Dura." Dev Cell 42(5): 445-461.