Endothelial cells form the interface between intravascular and extravascular compartments and serve as a selective barrier for the diffusion of cells and macromolecules between these two compartments. While endothelial cells lining blood vessels of the skin share features of endothelial cells elsewhere in the body, they also exhibit several skin specific characteristics. They actively participate in a variety of physiological processes including wound healing, control of hemostasis, temperature regulation, and modulation of inflammation/leukocyte trafficking.
Human Dermal Microvascular Endothelial Cells-adult (HDMEC-a) are isolated from human skin. HDMEC-a are cryopreserved at passage 1 and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HDMEC-a are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM), and by uptake of DiI-Ac-LDL.
HDMEC-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HDMEC-a are guaranteed to further expand for 15 population doublings under the conditions provided.
It is recommended to use Endothelial Cell Medium (ECM, Cat. No. SC1001) for culturing HDMEC-a in vitro.
HDMEC-a are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.