The choroid plexus is located in the ventricles of the brain where cerebrospinal fluid (CSF) is produced. It participates in brain development, maturation, aging, endocrine regulation, neuroimmune interactions, and pathogenesis of certain neurodegenerative diseases.
The choroid plexus consists of a network of capillaries enclosed by a single layer of epithelial cells that together form the blood-CSF barrier.
Capillaries in the choroid plexus contain a single layer of endothelial cells interrupted by “pores” which exhibit a diaphragm between the lumen and the interstitial space. Studies have shown that choroid plexus endothelial cells express high levels of Glut-1 glucose transporter, which supports the idea that epithelial and endothelial cells in the choroid plexus provide a metabolic work capability for maintaining ionic gradients and secretory functions across the blood-CSF barrier.
Human Choroid Plexus Endothelial Cells (HCPEC) are isolated from human brain.
HCPEC are cryopreserved after purification and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HCPEC are characterized by immunofluorescence with antibodies specific to VWF/Factor VIII, CD31 (PECAM), and by uptake of DiI-Ac-LDL.
HCPEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HCPEC are guaranteed to further expand for 10 population doublings under the conditions provided.
It is recommended to use Endothelial Cell Medium (ECM, Cat. no. SC1001) for culturing HCPEC in vitro.
HCPEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.