Human hepatocytes offered by 3H Biomedical are isolated from human liver by Biopredic International according to the method of Guguen-Guillouzo C and Guillouzo A (In“Isolated and cultured hepatocytes”, Guillouzo A. and Guguen-Guillouzo C, Eds. INSERM Paris & John Libbey Eurotext London, 1986, pp.1-12.).
Human hepatocytes are negative for HIV 1-2, HBV, HCV, mycoplasma, bacteria, yeast and fungi, and are characterized using the following functional controls:
The cells are seeded in a collagen I coated 6- / 12- / 24- / 48- / 96-well plate in Williams medium for hepatocyte seeding (Cat. no. BMIL212) and are cultured for a few hours in an incubator at 37°C and 95% air/5% CO2 before shipment at room temperature. The packaging warrants cell quality for a maximum of 2 days shipping.
The human hepatocytes are intended for immediate use. Culture the human hepatocytes in monolayer and short term culture at the conditions provided in the Instruction for use.
Hepatocyte incubation medium including:
Cat. no. BMIL600C, Basale hepatic cell medium, 100 ml
Cat. No. Add222C, Additives/supplements for hepatocyte incubation medium, 1 vial
Certificate of Analysis of cells and medium will be sent together with products.