HHMa from ScienCell Research Laboratories are isolated from humanliver. HHMa are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 106cells in 1 ml volume. HHMa are characterized by immunofluorescence with antibody to F4/80. HHMa are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHMa are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories; however, HHMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.
Recommended Medium: It is recommended to use MacrophageMedium (MaM, Cat. SC1921) for culturing HHMa in vitro.
Product Use: HHMa are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Liaskou E, Wilson DV, Oo YH. (2012) "Innate immune cells in liver inflammation." Mediators Inflamm. 2012:949157.[2] Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, Wouters K, Lütjohann D, Gijbels MJ, Febbraio M, Binder CJ, Hofker MH, Shiri-Sverdlov R. (2012) "Internalization of modified lipids by CD36 and SR-A leads to hepatic inflammation and lysosomal cholesterol storage in Kupffer cells." PLoS One. 7:e34378.[3]Tian Y, Jochum W, Georgiev P, Moritz W, Graf R, Clavien PA. (2006) "Kupffer cell-dependent TNF-alpha signaling mediates injury in the arterialized small-for-size liver transplantation in the mouse." Proc Natl Acad Sci U S A. 103:4598-603. [4] Seki E, de Minicis S, Inokuchi S, Taura K, Miyai K, van Rooijen N, Schwabe RF, Brenner DA.(2009) "CCR2 promotes hepatic fibrosis in mice." Hepatology. 50:185-97.