Cryopreserved undifferentiated HepaRG cells at P12 and the Progenitor cells can be expanded and differentiated for 2 to 3 months.
Master and working bank setup:
at least 1 month
Possibility of cell line manipulation
transfection, infection,…
Convenient for large scale projects.
HepaRG® cells can be used up to passage 20.
The differentiation process takes 4 weeks : 2 weeks in Growth medium and 2 weeks in Differentiation medium.
The differentiated cultures can then be used for up to 4 weeks.
HepaRG® cells gradually age and enter senescence – just like primary cells.
The HepaRG™ cell line needs specific media and additives for cell thawing, cell plating, maintenance, and differentiation.
Proliferation of HepaRG® cells Medium
BPMIL700 + ADD710* / ADD711**
Differentiation of HepaRG® cells Medium
BPMIL700 + ADD720/ ADD721
Applications
Drug Metabolism
Pre-induction
ADMETox
Toxicity endpoint applications.
How to obtain HepaRG cell line?
The HepaRG101 cell line is owned by Inserm as a material and covered by Inserm patents.
It is available under a License Agreement (for-profit customers or for academic projects funded in part or in full by for-profit organizations) or under an MTA (for nonprofit customers who are not funded neither in full nor in part by a for-profit organization) made with Biopredic on behalf of Inserm.