Smooth muscle contraction is the fundamental event in gastrointestinal motion. Inflammation of the human intestine causes increased levels of smooth muscle-specific actin, which in turn promotes the thickening of the smooth muscle layers. The increased smooth muscle actin may affect force production and further demonstrates the plasticity of smooth muscle cells in the inflamed intestine. The availability of HISMC makes it more feasible to study the contractile and proliferative tissue responses of human intestinal smooth muscle.
Human intestinal smooth muscle cells (HISMC) are isolated from human intestine.
HISMC are cryopreserved at passage 1 (P1) and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HISMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin and desmin.
HISMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
HISMC are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. no. SC1101) for culturing HISMC in vitro.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Product Use
HISMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.