HCF-a from ScienCell Research Laboratories are isolated from human adult heart. HCF-a are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCF-a are characterized by immunofluorescence with antibody specific to fibronectin. HCF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCF-a are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium: It is recommended to use Fibroblast Medium-2 (FM-2, Cat. SC2331) for culturing HCF-a in vitro.
Product Use: HCF-a are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References:  Sabri A, Short J, Guo J, Steinberg SF. (2002) "Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes." Circ Res. 91: 532-9. Akiyama-Uchida Y. (2002) "Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts." Hypertension. 40: 148-54. Burgess ML, Terracio L, Hirozane T, Borg TK. (2002) "Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts." Cardiovasc Pathol. 11: 78-87.