Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. Dermal fibroblasts can secrete large quantities of hyaluronan in response to inflammatory stimuli.
Human Primary Dermal Fibroblasts (HDF-f) are isolated from human skin. HDF-f are cryopreserved at P1 and delivered frozen on dry ice.
Each vial contains 500 000 cells in 1 ml volume.
Human Primary Dermal Fibroblasts are characterized by their spindle morphology and immunofluorescence with antibody specific to Fibronectin.
HDF-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HDF-f are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium:
It is recommended to use Fibroblast Medium (FM, Cat. no. SC2301) for culturing HDF-f in vitro.
Product Use
HDF-f are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.