Human Preadipocytes-subcutaneous (HPA-s) are isolated from human subcutaneous fat tissue. HPA-s are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. HPA-s are characterized by immunofluorescence with antibodies specific to CD44, CD90 and lipid staining after differentiation. HPA-s are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPA-s are guaranteed to further expand for 5 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Preadipocyte Medium (PAM, Cat. No. SC7211) for culturing HPA-s in vitro. Preadipocyte Differentiation Medium (PADM, Cat. No. SC7221) can be used for in vitro differentiation of preadipocytes into mature adipocytes, and then followed by Adipocyte Medium (AdM, Cat. No. SC7201), which maintains mature adipocytes after differentiation.
Product Use: HPA-s are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.