Luciferase Stable PC3 Cell Line

Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. The Luciferase stable PC3 Cell line was generated using abm's pLenti-III-UbC-Luciferase lentivirus on PC3 cells. The expression of luciferase has been confirmed with luciferase assay. All clones are puromycin (0.1 µg/ml)-resistant. Luciferase stable PC3 cells can be a very useful cell line for non-invasive visualization in in vitro experiments.

Quantity: 10⁶ cells/1.0 ml

Organism: Human

Source Organ: Adenocarcinoma; Prostate

BioSafety Level: II

Growth Properties: Adherent

Morphology: Epithelial-like

Clones: 13, 38

Population Doubling: 30-35 hours

Seeding Density: 30,000-45,000 cells/cm²; Recommended split ratio is 1:2 to 1:3

Applications: For Research Use Only

Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Shipping Conditions: Dry Ice

Storage Conditions: -180°C