Schwann cells are valuable tools for diabetic neuropathy pathogenesis involving glycation, reduced regenerative capability, oxidative stress, and other neurodegenerative diseases. The Immortalized Rat Schwann Cells express transcription factors (Krox20, Oct6, and SOX10), myelin proteins (P0, PMP22, and MAG), neurotrophic factors (NGF, GDNF, and CNTF), cell adhesion molecules (L1, NCAM, and N-Cadherin), and neurotrophin receptors (truncated TrkB, and TrkC), all of which points to distinct Schwann cell phenotype. Other glial markers such as S100, p75NTR, vimentin, GFAP, and laminin are also exhibited. These cells are unique in that they retain features of mature Schwann cells with the ability to myelinate neurites in co-culture with rat DRG neurons, thus providing a valuable tool to study neuron-Schwann cell interactions. Immortalization Method: Spontaneous ImmortalizationBioSafety Level: IISpecies: RatSource Organ: BrainGrowth Properties: AdherentMorphology: Spindle shapedPopulation Doubling: N/ASeeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, 20 ng/ml neuregulin-beta, 2 µM forskolin/DMSO, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1% Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Note: Growth of cells is dependent in cell density. The cells should be seeded in high density, otherwise their growth slows down.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Immunoreactivity to cell markersShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C