Ovarian granulosa cells play an important physiological role in supporting the development and selection of ovarian follicles by controlling oocyte maturation and by producing the steroid hormones, estradiol and progesterone, which are critical for maintenance of the ovarian cycle. The immortalized rat granulosa cells exhibit a temperature-dependent phenotype, with respect to morphology, growth, and the expression of prohibitin. RGA-1 cells will serve as a useful model in studies related to the functional relevance of prohibitin during cell differentiation and apoptosis.
Immortalization Method: Immortalized with a temperature-sensitive mutant (tsA209) of SV40
BioSafety Level: II
Organism: Rat (R. norvegicus)
Species: Rat
Source Organs: Ovary
Organ Type: Ovary
Growth Properties: Adherent/Suspension
Morphology: Elongated, overlapped and binucleated at +33°C or is mononucleated at 39°C.
Markers: PCNA
Donor Age: 21 days old
Donor Gender: Female
Propagation:Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 2.5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +33.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Western blot; 2) Northern blot; 3) Immunofluorescence
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Thompson, W. E., Branch, A., Whittaker, J. A., Lyn, D., Zilberstein, M., Mayo, K. E., & Thomas, K. (2001). Characterization of Prohibitin in a Newly Established Rat Ovarian Granulosa Cell Line. Endocrinology, 142(9), 4076-4085. doi:10.1210/endo.142.9.8354