Immortalized Rat Myoblast-like Tetracycline Cell Line (RMT 6211)

Immortalized Rat Myoblast-like Tertracycline Cell Line (RMT 6211) is derived from rat skeletal muscle and have been immortalized via transfection with the NIT-Tag retrovirus, containing a temperature sensitive mutant of the SV40 large T Antigen. This cell line was developed in order to provide a source of myofibers that could exhibit contractile activity similar to that seen in primary muscle cultures. Follwing differentiation, the RMT 6211 cell line was found to express markers for Pax7, MyoD, Myf-5, MRF4, and Myogenin. As a result, this immortalized cell line is a useful alternative to primary cultures and can be used to study muscle differentiation, as well as molecular and physiological aspects of electrical activity in muscle fibres.

Immortalization Method: Immortalized with NIT-Tag retrovirus containing a temperature sensititve SV40 Large T antigen

BioSafety Level: II

Organism: Rat

Species: Rat

Source Organs: Muscle

Organ Type: Muscle

Growth Properties: Adherent

Morphology: Fibroblast-like

Population Doubling: 14 - 21 hours

Markers: Pax7, MyoD, Myf-5, MRF4, and Myogenin

Donor Age: 6 months old

Propagation: The base medium for this cell line is Prigrow III medium, available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and horse serum* to a final concentration of 10%.Change media every 2-3 days.Carbon dioxide (CO₂): 8%, Temperature: +37.0°C.* Do not use heat-inactivated serum for cell culture unless specified otherwise.For differentiation, supplement Prigrow III media, Cat. No. TM003 with 5% heat-inactivated horse serum and 1 μg/ml dox.

Quality Control: 1) Immunofluorescence; 2) Western blotting

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C

Reference: Macpherson, P. C., Suhr, S. T., & Goldman, D. (2004). Activity-dependent gene regulation in conditionally-immortalized muscle precursor cell lines. Journal of cellular biochemistry, 91(4), 821–839. https://doi.org/10.1002/jcb.10784