Immortalized Rat Hypothalamus Cells (RCHT-1) express immunofluorescent-positive markers for dopamine
β-hydroxylase and the norepinephrine transporter, NET. The Immortalized Rat Hypothalamus Cells (RCHT-1) were derived from the hypothalamus of a 4-month-old Fisher 344 rat and were immortalized spontaneously in cell culture. When cultured
in vitro, the cells exhibit adherent growth properties and tend to grow in monolayers. The Immortalized Rat Hypothalamus Cells (RCHT-1) are useful in the field of neurology. Applications of this cell line include the study of dopamine and its role in neurological diseases.
Immortalization Method: Spontaneous immortalization
BioSafety Level: II
Organism: Normal Fisher 344 rat
Species: Rat
Source Organs: Hypothalamus
Organ Type: Brain
Growth Properties: Adherent
Morphology: Polygonal
Markers: Immunofluorescent-positive markers for dopamine
β-hydroxylase and the norepinephrine transporter, NET
Donor Age: 4 months old
Propagation: The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, 2 mM L-glutamine (G275), and 1% Penicillin/Streptomycin Solution (G255). Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Hurtado-Guzmán, C., Martínez-Alvarado, P., Dagnino-Subiabre, A., Paris, I., Caviedes, P., Caviedes, R., … Segura-Aguilar, J. (2002). Neurotoxicity of some MAO inhibitors in adult rat hypothalamic cell culture. Neurotoxicity Research, 4(2), 161–163. https://doi.org/10.1080/1029842029001594