Immortalized Rat Enteroglial Cells (EGC)

Enteroglial cells (EGCs) have a key role in the inflammatory response in the enteric nervous system which is structurally and functionally the largest and most diverse in complexity part of the peripheral nervous system. The Immortalized Rat Enteroglial Cells (EGC) is a valuable tool for studies in the enteric nervous system inflammatory response as it is an immortalized cell line established from the rat myenteric plexus. The cells express low levels of c-fos mRNA but can be up-regulated with stimuli from pro-inflammatory cytokines (IL-1β and TNF-α).

Immortalization Method: Serial passaging and infection by incubating with the supernatant of virus-producing GPE-egfr-neu cell line

BioSafety Level: II

Species: Rat

Source Organ: Jejunum

Growth Properties: Adherent

Morphology: Stellate shaped

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: Male

Donor Ethnicity: N/A

Propagation: Propagation: Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
To up-regulate c-fos mRNA levels: Stimulate cells for 60 minutes with either 10 ng/mL of IL-1β or 10 ng/mL of TNF-α.
Split ratio: 1:4 or 1:6
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Characterized by immunohistochemistry and dot-blot analysis.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C