Immortalized Rat Embryonic Striatal (M26-1F) Cells - tsSV40T

Immortalized Rat Embryonic Striatal (M26-1F) Cells - tsSV40T has GABAgeric properties with multiple nucleoli, presence of GAD and MAP-2 antigen, absence of vimentin, and no contact inhibition features. It is a tool which can be used to study GABA uptake and release, neural development and differentiation, and is a good model for Huntington’s disease. The cells grown in permissive temperature (33°C), whereas in non-permissive temperature (39.5°C), the morphology of the cells will become multipolar. SV40 large T antigen is stably expressed at both temperatures compared to the Immortalized Rat Embryonic Striatal (M213-2O) Cells - tsSV40T (available at abm, Cat. No. T0356).

Immortalization Method: Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen

BioSafety Level: II

Species: Rat

Source Organ: Straitum

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 53 - 63 hours

Seeding Density: 20,000 - 40,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Immunostaining for SV40 T large antigen and markers; 2) Test growth in permissive and non-permissive temperatures

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C