Immortalized Rat Arachnoid Cells - SV40/hTERT

The arachnoid cells are important in the maintenance of apical-basal polarity and the formation of tight junctions at the cerebrospinal fluid-blood barrier (CSF-blood barrier). The Immortalized Rat Arachnoid cells-SV40/hTERT derived from primary rat arachnoid cells maintained the ability to form intact epithelial monolayer and displayed normal immunohistochemical and morphological phenotypes. As such, the Immortalized Rat Arachnoid cell line is useful in physiological studies in modeling arachnoid granulations and cerebrospinal fluid flow. In addition, this cell line is also useful in pathological conditions such as hydrocephalus, arachnoid cysts, or other disorders.

Immortalization Method: Serial passaging and transduction with recombinant retroviruses carrying SV40 Large T antigen and hTERT gene

BioSafety Level: II

Species: Rat

Source Organ: Brain

Growth Properties: Adherent

Morphology: Spindle shaped|Cobble-stone

Population Doubling: approximately 35.3 hr

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: vimentin, cytokeratin and desmoplakin

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1. Immunofluorescence and Western Blotting were used to confirm the presence of SV40 (82kDa) and hTERT (122kDa) expression. 2. Immortalized Rat Arachnoid Cells exhibit spindle-like appearance at low density before reaching confluency and “cobblestone” appearance after becoming fully confluent, which appeared to be identical to its primary counterparts. 3. Immunofluorescence and Western Blotting were used to confirm the presence of arachnoid cell markers such as vimentin, cytokeratin and desmoplakin.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C