PAMs were isolated from ten 10-week-old clinically healthy Yorkshire pigs raised at a local pig farm. Primary porcine alveolar macrophages (PAM) are useful for studying viral infections and immune response in pigs. Immortalized PAM cells possessed the characteristics of primary PAMs, including strong expression of swine leukocyte antigen (SLA) class II genes and the inability to grow anchorage-independently. iPAM303 (73.3% ± 5.4; SLA DQA*0106/0201, DQB1*0202/0701, DRB1*0402/0602).
Immortalization Method: Immortalized with pBABE-SV40LT-Puro and pBABE-hTERT-Puro using PolyMag kit
BioSafety Level: II
Organism: Pig (Porcine)
Species: Pig (Porcine)
Source Organs: Lung
Organ Type: Lung
Growth Properties: Adherent
Morphology: Fibroblast-like
Markers: Puromycin resistance
Donor Age: 10 weeks old
Propagation:Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) PCR; 2) Immunohistochemistry; 3) Analysis of peptide binding to SLA class II molecules
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Van Chanh Le, Q., Le, T. M., Cho, H. S., Kim, W. I., Hong, K., Song, H., Kim, J. H., & Park, C. (2018). Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes. Veterinary research, 49(1), 96. https://doi.org/10.1186/s13567-018-0590-2