Immortalized MS^-/- Mouse Alveolar Macrophage Cells (ZK1)

Alveolar macrophages were isolated from the brochoalveolar lavage fluid of MARCO^-/- SR-AI/II^-/- (MS^-/- ) mice and were immortalized with the J2 retrovirus to establish the Immortalized MS^-/- Mouse Alveolar Macrophage Cells (ZK1). ZK1 cells exhibit the macrophage morphology, functional activity, and the ability to bind to and ingest particles are reduced due to the deficiency in MARCO and SR-AI/II scavenger receptors. It is a valuable tool to study the functions of MARCO and SR-AI/II scavenger receptors and particle toxicity.
abm also have available the ZK2 (T0677) and ZK6 (T0678)clones established from limiting dilution.

Immortalization Method: Infected with J2 retrovirus supernatants collected from the AMJ2-C11 cell line which carries the v­raf and v­myc oncogenes

BioSafety Level: II

Species: Mouse

Source Organ: Pulmonary Alveolus

Growth Properties: Adherent

Morphology: Round or Polygonal

Population Doubling: Doubling time of 14 hours

Seeding Density: 30,000 cells/cm²

Markers: F4/80 and CD11b

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow II medium available at abm (TM002). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) PCR genotyping used to determine the deficiency of MARCO and SR-AI/II; 2) Modified Wright staining used to identify macrophage morphology; 3) Macrophage-associated cell surface markers F4/80 and CD11b were determined by flow-cytometry.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C