Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT)

The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) were derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of control mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method. The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is able to retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, eNOS, VE-cadherin, and von Willebrand Factor. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro. These cells provide solution in difficulty in maintaining mouse aortic endothelial cells in multiple passages without phenotypic drift.

Immortalization Method: Immortalization using polyoma middle-sized T-antigen (PmT)

BioSafety Level: II

Species: Mouse

Source Organ: Heart

Growth Properties: Adherent

Morphology: Elongated

Population Doubling: N/A

Seeding Density: 20,000-40,000 cells/cm²

Markers: PECAM1, eNOS, VE-cadherin, and von Willebrand Factor

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.


Recommended split ratio of 1:2. Perform a PBS wash twice before using warmed 0.25% Trypsin-EDTA (TM050) to subculture. Cells must be seeded over 20,000 cells/cm². Cell seeded under this density will result a significant reduction in cell propagation.

Quality Control: 1) FACS cell sorting; 2) Characterization by Dil-Ac-LDL staining and immunostaining

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C