The Immortalized Mouse Urogenital Sinus Mesenchymal Cells (UGSM-2) are derived from a subline of the INK4a mouse, a transgenic knockout that lacks p16INK4a and p19ARF, which are specific inhibitors of cyclin-dependent kinases Cdk4 and Cdk6 that regulate cell cycle progression. UGSM-2 cells display a myofibroblast phenotype in culture with vimentin and smooth muscle actin expression, and is shown to respond to androgen stimulation. Co-culture of UGSM-2 cells with human prostate epithelium cells BPH-1, or co-grafting in vivo results in organized clusters of BPH-1 cells surrounded by a mantle of the UGSM-2 cells. The cells are responsive to Sonic hedgehog (SHH), an important signaling factor in prostate development, and mimic the transcriptional response of the intact UGS mesenchyme. This cell line is a useful model for studying the prostate stroma-epithelium signalling way, which plays an important role in prostate development and cancer progression.
Immortalization Method: p16INK4a and p19ARF Knockout
BioSafety Level: II
Species: Mouse
Source Organ: Prostate
Growth Properties: Adherent
Morphology: Spindle shaped|Fibroblast-like
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Gentamicin (50ug/ml) + 1% ITS + Glutamine (1x) + 10-8M DHT, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: Real Time PCR was used to shows activation of SHH target genes
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C