Description: The temporomandibular joint (TMJ) is the jaw joint that hinges to allows complex movements related to eating and swallowing. TMJ disc tissue permits mandibular movement and its degeneration has been linked to many clinical pathologies related to joint function, including crepitus, arthritis and headaches. TMJ disc tissue comprises of a complex matrix of cells with regional difference in morphology, including chondrocyte -like cells and fibroblast-like cells. This organization of cells allow for the dissipation of forces acting on the jaw joint. The immortalized Mouse TMJ Disc cell line was derived from Chondrocyte-Like Cells from a 12-week-old female mouse. These cells are designed to be suitable for cell-type specific studies on the physiology and progression of TMJ disc disorders. Immortalization Method: Immortalized with pGNR145 expressing hTERTBioSafety Level: IISpecies: MouseSource Organ: CartilageGrowth Properties: AdherentMorphology: Multi-polarPopulation Doubling: Approximately 24 hoursSeeding Density: 5,000 - 10,000 cells/cm10²Markers: Chondrocyte marker COMP, collagen XDonor Age: 12 weeks oldDonor Gender: FemaleDonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow VIII medium available at abm, Cat. No. TM018. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) Western blot; 2) qRTPCR; 3) ImmunofluorescenceShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C