The temporomandibular joint (TMJ) is the jaw joint that hinges to allows complex movements related to eating and swallowing. TMJ disc tissue permits mandibular movement and its degeneration has been linked to many clinical pathologies related to joint function, including crepitus, arthritis and headaches. TMJ disc tissue comprises of a complex matrix of cells with regional difference in morphology, including chondrocyte -like cells and fibroblast-like cells. This organization of cells allow for the dissipation of forces acting on the jaw joint. The immortalized Mouse TMJ Disc cell line was derived from Chondrocyte-Like Cells from a 12-week-old female mouse. These cells are designed to be suitable for cell-type specific studies on the physiology and progression of TMJ disc disorders.
Immortalization Method: Immortalized with pGNR145 expressing hTERT
BioSafety Level: II
Species: Mouse
Source Organ: Cartilage
Growth Properties: Adherent
Morphology: Multi-polar
Population Doubling: Approximately 24 hours
Seeding Density: 5,000-10,000 cells/cm²
Markers: Chondrocyte marker COMP, collagen X
Donor Age: 12 weeks old
Donor Gender: Female
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow VIII medium available at abm, Cat. No. TM018. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Western blot; 2) qRTPCR; 3) Immunofluorescence
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C