TαT-1 cells were derived from a transgenic mice, immortalized by the SV40T antigen. It expresses β1 and β2 thyroid hormone receptors as well as retinoid X receptor-γ. These TαT-1 cells are useful as a model for further studies in the cis and trans-acting elements of TSH expression.
Immortalization Method: N/A
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: Mouse
Source Organs: Pituitary Gland
Organ Type: Pituitary Gland
Growth Properties: Adherent
Markers: αGSU and TSHb genes
Propagation: Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at
abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) RT-PCR
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Alarid, E. T., Windle, J. J., Whyte, D. B., & Mellon, P. L. (1996). Immortalization of pituitary cells at discrete stages of development by directed oncogenesis in transgenic mice. Development (Cambridge, England), 122(10), 3319–3329.Alarid, E. T., Holley, S., Hayakawa, M., & Mellon, P. L. (1998). Discrete stages of anterior pituitary differentiation recapitulated in immortalized cell lines. Molecular and cellular endocrinology, 140(1-2), 25–30. https://doi.org/10.1016/s0303-7207(98)00025-2Yusta, B., Alarid, E. T., Gordon, D. F., Ridgway, E. C., & Mellon, P. L. (1998). The thyrotropin beta-subunit gene is repressed by thyroid hormone in a novel thyrotrope cell line, mouse T alphaT1 cells. Endocrinology, 139(11), 4476–4482. https://doi.org/10.1210/endo.139.11.6283