Previously immortalized sertoli cells- involved in androgen regulation during spermatogenesis- lose androgen receptor (AR) expression or androgen responsiveness; thus leaving them ill-suited for any further research regarding androgen regulation. The Immortalized Mouse Sertoli Cells (ST38c) eliminate this barrier with their continuous expression of endogenous AR. These cells are perfectly suited for investigating androgen receptor signaling in sertoli cell function in order to determine both molecular and cellular mechanisms.
Immortalization Method: Isolated from a transgenic mouse carrying SV40 large T Antigen under the control of the human vimentin promoter
BioSafety Level: II
Species: Mouse
Source Organ: Testes
Growth Properties: Adherent
Morphology: Spindle shaped
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: Male
Donor Ethnicity: N/A
Propagation: ST38c cells must be grown in Prigrow III (TM003) base medium supplemented with a final concentration of fetal bovine serum (TM999)* to a final concentration of 5% and a final concentration of Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1. Androgen receptor mRNA was confirmed via RT-PCR while western blot confirmed AR protein expression. 2. Androgen receptor regulated genes were identified via microarray assays. 3. SV40 Tag expression confirmed through fluorescence microscopy
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C