Schwann cells are valuable tools for diabetic neuropathy pathogenesis involving glycation, reduced regenerative capability, oxidative stress, and other neurodegenerative diseases. The Immortalized Mouse Schwann Cells express glial cell markers (S100, GFAP, p75NTR); transcription factors involved in Schwann cell development and peripheral myelin formation (Krox20, Oct6, PAX3, and SOX10); and neurotrophic factors (NGF, BDNF, GDNF, and CNTF). In the presence of growth factors, such as PDGF-BB, aFGF, bFGF, or TGF-β1, these cells exhibit mitogenic reponses. The IMS32 cells may be used to study action mechanisms involved in peripheral nerve regeneration, and as an in vitro model to assess the pathogenesis of neurological diseases. Immortalization Method: Spontaneous ImmortalizationBioSafety Level: IISpecies: MouseSource Organ: BrainGrowth Properties: AdherentMorphology: Spindle shapedPopulation Doubling: N/ASeeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Note: Growth of cells is dependent on cell density. The cells should be seeded in high density, otherwise their growth slows down.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Immunoreactivity to cell markersShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C