IKARS1 was immortalized spontaneously from AR-deficient female mice. Aldose reductase (AR) is involved in the enzymatic conversion of glucose into sorbitol in the polyol pathway. Hyperactivity in this pathway caused by high levels of glucose in the bloodstream increases the effects of AR, leading to diabetic nerve damage known as diabetic neuropathy. This AR-deficient cell line can be used in studies involving pathways affected by diabetic conditions.
Immortalization Method: Spontaneous
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: Mouse
Source Organs: Brain
Organ Type: Brain
Growth Properties: Adherent
Morphology: Spindle-shaped
Markers: Neo drug resistant marker
Quality Control: 1)Immunocytochemistry; 2) Western Blot; 3) Neurite outgrowth and neuronal cell survival assays; 4) ELISA; 5) LC/MS/MS; 6) Microarray analysis; 7) Real-time RT-PCR
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Niimi, N., Yako, H., Takaku, S., Kato, H., Matsumoto, T., Nishito, Y., Watabe, K., Ogasawara, S., Mizukami, H., Yagihashi, S., Chung, S. K., & Sango, K. (2018). A spontaneously immortalized Schwann cell line from aldose reductase-deficient mice as a useful tool for studying polyol pathway and aldehyde metabolism. Journal of neurochemistry, 144(6), 710–722. https://doi.org/10.1111/jnc.14277