Immortalized Mouse Schwann Cells

Immortalized Mouse Schwann Cells are derived from Twitcher mice. The Schwann cells were immortalized spontaneously in culture, dubbed as the TwS1 cells, and can be used as a murine model for globoid cell leukodystrophy (Krabbe disease) studies. Unlike the normal mouse Schwann cells (Immortalized Mouse Schwann Cells (IMS32), T0295), the TwS1 cells have a nonsense mutation in the GALC genome which is associated with the pathogenesis of Krabbe disease. These cells also exhibit markers for S100, laminin, p75 ^NTR, GFAP and vimentin.

Immortalization Method: Spontaneous immortalization

BioSafety Level: II

Species: Mouse

Source Organ: Brain

Growth Properties: Adherent

Morphology: Spindle shaped

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
The growth of TwS1 cells is cell density-dependent. The cells should be seeded in rather high density, otherwise their growth becomes very slow.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Immunofluorescence detection for S100, laminin, and p75 ^NTR, and for GFAP and vimentin markers

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C