Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 were generated through the infection of freshly harvested mouse bone marrow cells with the LRARα403SN retroviral vector. Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40, when treated with retinoic acid, terminally differentiate into mature neutrophils. Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 are GM-CSF dependent. Research uses of Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 include studying the effect of retinoic acid in the differentiation of neutrophils.
Immortalization Method: Immortalized with retroviral vector LRARα403SN
BioSafety Level: II
Species: Mouse
Source Organ: Bone Marrow
Growth Properties: Suspension
Morphology: Promyelocyte characteristics; tend to form aggregates
Population Doubling: N/A
Seeding Density: N/A
Markers: Express high levels of retroviral mRNA harboring the truncated RARα403 sequence; mouse neutrophil-specific antigen 7/4; positive staining for chloroacetate esterase
Donor Age: Six weeks old
Donor Gender: Male
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow V medium available at abm, Cat. No. TM015. To make the complete growth medium, add the following components to the base medium: horse serum* to a final concentration of 20%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, WEHI-3B conditioned medium to a final concentration of 20%, Polybrene (G062) to a final concentration of 4 µg/ml, Recombinant Mouse GM-CSF (CSF2) (Z200075) to a final concentration of 2.5 ng/ml, Recombinant Human IL1B (Z100395) to a final concentration of 10 ng/ml, and Recombinant Human IL6 (Z100555) to a final concentration of 20 ng/ml. Change media every 2-3 days. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated serum for cell culture unless specified otherwise.
Quality Control: 1) Growth pattern analysis; 2) Northern analysis; 3) Transient expression analysis
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C