Immortalized Mouse Pituitary Progenitor Cells (αT1-1) represent a progenitor to the gonadotrope and/or thyrotrope lineages generated by directing expression of the oncogene SV40 T antigen using a gonadotrope-specific region of the human glycoprotein hormone α-subunit gene in transgenic mice. Immortalized Mouse Pituitary Progenitor Cells (αT1-1) provide a novel model system in which the molecular mechanism of pituitary cellular differentiation can be dissected.
Immortalization Method: Immortalized with the SV40 T antigen
BioSafety Level: II
Organism: Mouse
Species: Mouse
Source Organs: Pituitary
Organ Type: Pituitary
Growth Properties: Adherent
Morphology: Polygonal
Markers: Cga, human glycoprotein hormone α-subunit
Propagation: Use of Matrigel-coated plates (Collaborative Biomedical Products) is required for cell adhesion to the culture vessels. Grow cells in Matrigel-coated plates with the following conditions.The base culture medium for this cell line is Prigrow III medium available at
abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Pituitary cell differentiation; 2) Pituitary transcription factor expression
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Alarid, E. T., Windle, J. J., Whyte, D. B., and Mellon, P. L. (1996). Immortalization of Pituitary Cells at Discrete Stages of Development by Directed Oncogenesis in Transgenic Mice. Development 122, 3319-3329