Immortalization Method: N/A
BioSafety Level: II
Organism: Mouse
Species: Mouse
Source Organs: Liver
Organ Type: Liver
Growth Properties: Adherent
Morphology: Epithelial-like
Population Doubling: 24-34 hours
Propagation:Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Proell, V., Mikula, M., Fuchs, E., & Mikulits, W. (2005). The plasticity of p19 ARF null hepatic stellate cells and the dynamics of activation. Biochimica et biophysica acta, 1744(1), 76–87. https://doi.org/10.1016/j.bbamcr.2004.12.009Mikula, M., Proell, V., Fischer, A. N., & Mikulits, W. (2006). Activated hepatic stellate cells induce tumor progression of neoplastic hepatocytes in a TGF-beta dependent fashion. Journal of cellular physiology, 209(2), 560–567. https://doi.org/10.1002/jcp.20772Proell, V., Carmona-Cuenca, I., Murillo, M. M., Huber, H., Fabregat, I., & Mikulits, W. (2007). TGF-beta dependent regulation of oxygen radicals during transdifferentiation of activated hepatic stellate cells to myofibroblastoid cells. Comparative hepatology, 6, 1. https://doi.org/10.1186/1476-5926-6-1Sancho, P., Mainez, J., Crosas-Molist, E., Roncero, C., Fernández-Rodriguez, C. M., Pinedo, F., Huber, H., Eferl, R., Mikulits, W., & Fabregat, I. (2012). NADPH oxidase NOX4 mediates stellate cell activation and hepatocyte cell death during liver fibrosis development. PloS one, 7(9), e45285. https://doi.org/10.1371/journal.pone.0045285He, S., Li, Y., Chen, Y., Zhu, Y., Zhang, X., Xia, X., & Sun, H. (2015). Immortalization of pig fibroblast cells by transposon-mediated ectopic expression of porcine telomerase reverse transcriptase. Cytotechnology, 68(4), 1435–1445. doi:10.1007/s10616-015-9903-8Lenk, L., Pein, M., Will, O., Gomez, B., Viol, F., Hauser, C., Egberts, J. H., Gundlach, J. P., Helm, O., Tiwari, S., Weiskirchen, R., Rose-John, S., Röcken, C., Mikulits, W., Wenzel, P., Schneider, G., Saur, D., Schäfer, H., & Sebens, S. (2017). The hepatic microenvironment essentially determines tumor cell dormancy and metastatic outgrowth of pancreatic ductal adenocarcinoma. Oncoimmunology, 7(1), e1368603. https://doi.org/10.1080/2162402X.2017.1368603Knaack, H., Lenk, L., Philipp, L. M., Miarka, L., Rahn, S., Viol, F., Hauser, C., Egberts, J. H., Gundlach, J. P., Will, O., Tiwari, S., Mikulits, W., Schumacher, U., Hengstler, J. G., & Sebens, S. (2018). Liver metastasis of pancreatic cancer: the hepatic microenvironment impacts differentiation and self-renewal capacity of pancreatic ductal epithelial cells. Oncotarget, 9(60), 31771–31786. https://doi.org/10.18632/oncotarget.25884