The olfactory bulb is located within the vertebrate forebrain, in which it functions to receive nerve endings/input from the nasal cavity. The sense of smell is associated with the nerve tissues of the olfactory bulb.
Immortalized Mouse Olfactory Bulb Cells (OBC13) is immortalized with non-replicating retrovirus carrying the avian Myc gene. It is a sister derivative from the Immortalized Mouse Olfactory Bulb Cells (OBC1) (T0235). The cells exhibit GalC (a marker for oligodendroglial lineage) marker, but has the absence of NF (neurofilament marker). Voltage-dependent potassium channels are expressed as well. OBC13 cells have been shown to form tumors in animal models, hence has the ability to be tumorigenic. It is a valuable in vitro tool for studies relating to the functions and the biology of the olfactory bulb.Immortalization Method: Infection with retrovirus containing the avian Myc gene (pneoMLV)BioSafety Level: IISpecies: MouseSource Organ: Olfactory bulbsGrowth Properties: AdherentMorphology: Flattened|Dendritic processesPopulation Doubling: 23-33 hoursSeeding Density: 2,500 - 20, 000 cells/cm²Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: Grow cells in Poly-L-Lysine (TM061)-coated flasks with the following conditions. The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, 0.3 mg/ml G418 (G271), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) Resistance to 0.3 mg/ml G418, 2) Southern or Northern blot analysis of the viral integration site, 3) immunocytochemical analysis for markers Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C