Immortalized Mouse NPCNIH Schwann Cells (573C10)

Immortalized Mouse NPCNIH Schwann Cells (573C10) is a spontaneously immortalized cell line established from homozygous npc^nih/npc^nih mice. NPC1 is a gene responsible for the majority of Niemann–Pick disease type C (NPC) patients. NPC1 protein was not detectable in homozygous 573C10 cell. 573C10 Schwann cell line can be useful to investigate the pathomechanism of nervous system lesions in NPC, the therapeutic approaches against neurological deficits in patients with NPC and, more in general, the biology of cholesterol, sphingolipid and fatty acid transports in the nervous system.

Immortalization Method: Spontaneous immortalization

BioSafety Level: II

Species: Mouse

Source Organ: Brain

Growth Properties: Adherent

Morphology: N/A

Population Doubling: N/A

Seeding Density: N/A

Markers: S100, laminin, p75NTR, GFAP, GM2 ganglioside, filipin cholesterol cytochemistry

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Cytological analysis; 2) RT-PCR; 3) Western blot

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C