Immortalized Mouse Neuronal Cells (M4b)

The Immortalized Mouse Neuronal Cells (M4b) were derived from the spinal cord of a fetal mouse after 12-16 days of gestation. The fetal mouse was bred from double heterozygous (Rb 2H/RB 32 Lub) males and normal C57BL females. Transformation of the cell line was induced via supplementation with UCHT1 rat thyroid cell line-conditioned medium and was confirmed via the detection of vimentin. Immunohistochemistry found the presence of neurological makers, but the absence of glial markers in the Immortalized Mouse Neuronal Cells (M4b). In addition, the intracellular [Ca²⁺] response after neurotransmitter stimulation was rapid and transient as expected. The Immortalized Moue Neuronal Cells (M4b) is useful in studies of neuronal cell biology, viral infections, and neuronal cytotoxicity. The Immortalized Moue Neuronal Cells (M4b) can also be used as a control for the Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) (Cat. T0675), which is a mouse model of Down Syndrome.

Immortalization Method: Supplementation of plating medium with UCHT1 rat thyroid cell line-conditioned medium

BioSafety Level: II

Species: Mouse

Source Organ: Spinal Cord

Growth Properties: Adherent

Morphology: Neuronal

Population Doubling: 14-24 hours

Seeding Density: 40,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, Bovine Serum (Sigma) to a final concentration of 10%, 1% L-glutamine (G275) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) RT-PCR for SOD-1 and GAPDH; 2) Immunohistochemistry of neuronal markers; 3) Intracellular Ca²⁺ measurements;

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C