The Immortalized Mouse Macrophage Cells (BMA3.1A7) are derived the bone marrow of adult female C57BL/6 mice. These cells are spontaneously immortalized via over expression of raf and myc oncogenes and posses a “fried egg” morphology, characterized by a rounded appearance with a high number of vesicles. In addition, BMA3.1A7 has been shown to possess similar surface marker expression as bone marrow-derived macrophages (BMDM) such as iNOS, Arginase-1, CD86, MHC I, MHC II and CD206, which contribute to its use as a substitute for primary cells. Their similarity to these macrophages is very useful given that the plasticity of macrophages contributes to their varied pathogen response. In addition, the polarization that BMA3.1A7 undergoes allows it to alter its susceptibility to viral infection, making it a useful model to study the effects of polarization on viruses.
Immortalization Method: Spontaneous
BioSafety Level: II
Species: Mouse
Source Organ: Blood
Growth Properties: Adherent
Morphology: Irregular
Population Doubling: N/A
Seeding Density: N/A
Markers: iNOS, Arginase-1, CD86, MHC I, MHC II and CD206
Donor Age: Adult
Donor Gender: Female
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, L-glutamine (G275) to a final concentration of 2 mM, β-mercaptoethanol to a final concentration of 55 µM, MEM Non-Essential Amino Acids Solution to a final concentration of 1X, and HEPES to a final concentration of 10 mM. Change media every 2-3 days. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Flow cytometry; 2) Arginase assay; 3) Nitric oxide assay; 4) qRT-PCR
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C