Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1)

The Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1) are non-transformed cells derived from leptin-receptor (LepR) deficient mice which display some phenotypic and functional properties similar to primary macrophages. The cells are characterized as early differentiated macrophages since the high expression of MAC-2, and low expression of F4/80, Ly6G and Ly6C were observed; but CD11b expression was absent. It is recommended for use in studies involving the role of the leptin receptor, susceptibility to infections, innate immune functions, and obesity.

Immortalization Method: Spontaneous immortalization

BioSafety Level: II

Species: Mouse

Source Organ: Bone Marrow

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: Male

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: a final concentration of fetal bovine serum (TM999)* to a final concentration of 10%, 10% Opti-MEM (gibco), 15 mM/L HEPES, and 100 µg/ml gentamycin or Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) qRT-PCR confirmed LepR deficiency; 2) Flow cytometry was used to analyze cell surface markers.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C