Immortalized Mouse Intestinal Crypt Stem Cells (iMICs)

Immortalized Mouse Intestinal Crypt Stem Cells (iMICs) are immortalized through infection with non-replicating retrovirus carrying the loxP-flanked SV40 large T antigen. In vitro, the cells can be genetically manipulated for further research. Thus it is a reliable tool for research in mouse intestinal crypt biology.

Immortalization Method: Serial passaging and infection with retrovirus carrying loxP-flanked SV40 large T antigen

BioSafety Level: II

Species: Mouse

Source Organ: Intestine

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C