Immortalized Mouse Hexb^-/- Schwann Cells (1113C1)

Description: Immortalized Mouse Hexb^-/- Schwann Cells (1113C1) were spontaneously immortalized from dorsal root ganglia and peripheral nerves of Sandhoff mice. 1113C1 is genetically homozygous for the disrupted Hexb gene. This cell line can be used as a murine model for Sandhoff disease studies.

Immortalization Method: Spontaneous immortalization

BioSafety Level: II

Species: Mouse

Source Organ: Brain

Growth Properties: Adherent

Morphology: Spindle-shaped

Population Doubling: N/A

Seeding Density: N/A

Markers: S100, laminin, p75NTR, GFAP, GM2 ganglioside

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) RT-PCR; 2) Immunocytochemical analysis; 3) Enzyme assays and protein determination

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C