Immortalized Mouse Hepatocyte SIMH (CLOCK) Cells

The Immortalized Mouse Hepatocyte SIMH^CLOCK Cells contain a bioluminescent marker gene that reports on the circadian phase of the cells. The presence of the Per2:Luc reporter gene allows real time monitoring of clock phase. The major characteristics of this cell line include its high metabolic capacity, liver-like properties, robust synchronizable circadian clock, and capacity to stably express additional marker. When compared with other established cell lines (i.e. HepG2), the SIMH^CLOCK cells show a significantly better metabolic and xenobiotic capacity. This ability of the Immortalized Mouse Hepatocyte SIMH^CLOCK Cells is useful in studying treatment of liver cells with various physical or chemical compounds at defined stages of the circadian cycle and analyze the time-of-day exposure effects.

Immortalization Method: Transfection with mammalian expression vector carrying SV40 gene

BioSafety Level: II

Species: Mouse

Source Organ: Liver

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: Male

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IX medium available at abm (TM019). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Bioluminescence recording of luciferase activity; 2) Apoptosis assay using exposure to B[a]P; 3) qPCR; 4) RNA Seq analysis

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C