The Immortalized Mouse Hepatic Stellate Cells – SV40 were derived from C57/Bl6 WT mouse via enzymatic digestion and Percoll density gradient centrifugation. The primary cells were immortalized via transfection of the pCMV Simian Virus 40 Large T antigen and hygromycin B selection. The Immortalized Mouse Hepatic Stellate Cells – SV40 are useful in studies focusing on liver fibrosis since it is the major cell type involved in the formation of scar tissue. The cells are particularly applicable in studies associated with inflammatory responses, as NF-κB and inflammatory cytokines levels have been found to change in the Immortalized Mouse Hepatic Stellate Cells upon LPS stimulation.
Immortalization Method: Transfection of pCMV Simian Virus 40 Large T antigen
BioSafety Level: II
Species: Mouse
Source Organ: Liver
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 35-45 hours
Seeding Density: 20,000-40,000 cells/cm²
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% with Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +33.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Genotype analysis; 2)NF-κB Activation Assay; 3) Cytokine ELISA Assays; 4) 3[H]-thymidine Cell Proliferation Assay
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C